One in four UK newly diagnosed cancers in men is prostate cancer.
Last year, the body which regulates screening in the United Kingdom advised against routine screening.
The UK National Screening Committee said this study provided further evidence that the harms outweigh the benefits.
Prostate cancer kills 10,000 people each year in the United Kingdom.
Although there is no screening programme, requests men above 50 still a test.
ScreeningThis latest study was conducted in Norrkoping in Sweden. The 9,026 men who in their fifties or sixties in 1987 were followed.
???Continue reading the main storyconsiderably the potential harms outweigh the benefits of screening "end quote Dr. Anne Mackie UK National Screening Committee nearly 1,500 men were randomly chosen to be screened every three years between 1987 and 1996. The first two tests were conducted by digital rectal examination and then by testing of prostate-specific antigen.
The report concludes: "after 20 years of follow-up, the rate of death from prostate cancer does not differ significantly between men in the Group screening and those in the control group."
The favoured method of screening is the prostate-specific antigen (PSA) test.
However, about 15% of men with normal PSA levels will have prostate cancer and two-thirds of men with a high level of PSA in fact no prostate cancer.
A study has suggested that in order to prevent a death from prostate cancer you should screen 1,410 men and 48 of them treat.
Dr. Anne Mackie, Director of the programmes of the UK National Screening Committee, said: "this evidence offers further support for the recommendation of the Commission in November not to screen for prostate cancer created at this time.
"At this moment that the potentially harmful significantly outweigh the benefits of screening. We will assess the evidence for prostate cancer screening against our criteria again in three years, or earlier if it justifies new knowledge. "
Dr Sarah Cant, head of policy and campaigns for the Prostate Cancer Charity, said: "while this research suggests that screening men for prostate cancer is not the number of men die from the disease reduce, this was a relatively small study and not all rounds of the screening used the PSA test, which is the most effective test we have at present to indicate the prostate problems that may be cancer.
"We know of another larger study that using the PSA screening test can reduce mortality.
"This previous process demonstrated, however, that screening can lead to many men who an unnecessary treatment for a harmless prostate cancer. The Prostate Cancer Charity, therefore, does not believe there is enough evidence yet to support a screening program. "
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